Difference between revisions of "Part:BBa K1616023"

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Revision as of 16:17, 22 September 2015

T7 promoter - HisTag- NanoLuc - T7 terminator NanoLuc (Nluc) is a novel engineered luciferase coupled with a novel coelenterazine analogue: Furimazine. When both are coupled, we obtain a much brighter luminescence than Fluc or Rluc and it is known to work exceptionally well as a reporter. NanoLuc is a 19.1 kDa enzyme which is an ATP independent luciferase providing a glow-type luminescence.

Thermal stable enzyme NanoLuc retains activity following 30 min incubation at 55 °C. Active over broad pH range NanoLuc is fully active between pH 7-9 and retains significant activity at pH 5-7. Monomeric enzyme NanoLuc facilitates use as transcriptional reporter or fusion partner.

NanoLuc.png



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 117
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 20


Design Notes

We checked for all illegal restriction sites.

Source

NanoLuc is a 19.1 kDa enzyme which is an ATP independent luciferase providing a glow-type luminescence.

References

Kyle Hooper, Ph.D, Applications of a smaller, brighter, more versatile luciferase:NanoLuc™ Luciferase Technology, 2012
Shreeram Akilesh, Bioluminescence: Nature’s Bright Idea,2000.