Difference between revisions of "Part:BBa K1859021:Experience"
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===Applications of BBa_K1859021=== | ===Applications of BBa_K1859021=== | ||
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===User Reviews=== | ===User Reviews=== | ||
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| + | <html> | ||
| + | <p> | ||
| + | <a href= "http://2015.igem.org/Team:Gifu" > [iGEM gifu 2015] | ||
| + | </a> . | ||
| + | </p> | ||
| + | <p> | ||
| + | We inserted this generator into <i>E. coli</i> K-12 JM109.<br> | ||
| + | We cultivated this bacteria and performed SDS-PAGE without boiling.<br> | ||
| + | Lane F shows this sample.<br><br> | ||
| + | </p> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/0/04/Gifu-sds-before.jpeg" width="" height="240" vspace="10" hspace="50" align=""></img> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/a/ab/Gifu-sds-dye.jpg" width="" height="240" vspace="10" hspace="50" align=""></img> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/6/60/Gifu-sds-page-combine.png" width="" height="240" vspace="10" hspace="50" align=""></img> | ||
| + | <br>left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay | ||
| + | <br><br> | ||
| + | <p> | ||
| + | There was no fluorescence. We found that this generator doesn’t make functional long chain protein.<br> | ||
| + | </p> | ||
| + | </html> | ||
Revision as of 09:54, 22 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1859021
User Reviews
UNIQebacb6000aeb8336-partinfo-00000000-QINU UNIQebacb6000aeb8336-partinfo-00000001-QINU
We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane F shows this sample.
left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay
There was no fluorescence. We found that this generator doesn’t make functional long chain protein.