Difference between revisions of "Part:BBa K1859020:Experience"
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+ | <p> | ||
+ | <a href= "http://2015.igem.org/Team:Gifu" > [iGEM gifu 2015] | ||
+ | </a> . | ||
+ | </p> | ||
+ | <p> | ||
We inserted this generator into <i>E. coli</i> K-12 JM109.<br> | We inserted this generator into <i>E. coli</i> K-12 JM109.<br> | ||
We cultivated this bacteria and performed SDS-PAGE without boiling.<br> | We cultivated this bacteria and performed SDS-PAGE without boiling.<br> | ||
Lane E shows this sample.<br><br> | Lane E shows this sample.<br><br> | ||
+ | </p> | ||
<img src="https://static.igem.org/mediawiki/2015/0/04/Gifu-sds-before.jpeg" width="" height="240" vspace="10" hspace="50" align=""></img> | <img src="https://static.igem.org/mediawiki/2015/0/04/Gifu-sds-before.jpeg" width="" height="240" vspace="10" hspace="50" align=""></img> | ||
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<br>left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay | <br>left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay | ||
<br><br> | <br><br> | ||
− | There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.< | + | <p> |
− | + | There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.</p> | |
− | + | ||
− | + | ||
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</html> | </html> |
Revision as of 09:42, 22 September 2015
This experience page is provided so that any user may enter their experience using this part.
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UNIQb4003052273ed079-partinfo-00000000-QINU UNIQb4003052273ed079-partinfo-00000001-QINU
We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane E shows this sample.
left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay
There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.