Difference between revisions of "Part:BBa K1859024"
 
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<p>
 
<p>
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mRNA circularize efficiently generator (outside complementarity)
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mRNA circularize efficiently generator (inside complementarityⅠ)
 
</p>
 
</p>
  
 
<p>
 
<p>
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We designed this generator [K1859024] to improve circular efficiency of mRNA including His-RFP sequence.
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We designed this generator [BBa_K1859024] to improve circular efficiency of mRNA including His-RFP sequence.
 
</p>
 
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859004" > [BBa_K1859004] </a>
 
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859004" > [BBa_K1859004] </a>
 
), His-RFP without stop codon
 
), His-RFP without stop codon
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_BBa_K1332002" >[BBa_K1332002] </a>
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332002" >[BBa_K1332002] </a>
 
, Lacl
 
, Lacl
 
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_R0010] </a>
 
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_R0010] </a>
 
, RBS
 
, RBS
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0034" >[BBa_B0034] </a>
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034" >[BBa_B0034] </a>
 
  and DT
 
  and DT
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_B0015] </a>
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_B0015" >[BBa_B0015] </a>
 
.
 
.
 
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We found that circular efficiency of [BBa_1859024] was the third, when we compared with amount of circular mRNA made by these generators  
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We found that circular efficiency of [BBa_K1859024] was the third, when we compared with amount of circular mRNA made by these generators  
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332011" > [BBa_1332011] </a>
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332011" > [BBa_K1332011] </a>
 
, [BBa_1859024],  
 
, [BBa_1859024],  
 
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859025" > [BBa_K1859025] </a>
 
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859025" > [BBa_K1859025] </a>

Latest revision as of 09:41, 22 September 2015

mRNA circularize efficiently generator (inside complementarityⅠ)

We designed this generator [BBa_K1859024] to improve circular efficiency of mRNA including His-RFP sequence.

The generator is comprised of Circular parts combined the linker( [BBa_K1859003] and [BBa_K1859004] ), His-RFP without stop codon [BBa_K1332002] , Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] .

Linkers designed to adjust folding of protein are in [BBa_K1859003] and [BBa_K1859004] . RNA sequences of these linkers bind complementary with each other in circular mRNA.

We found that circular efficiency of [BBa_K1859024] was the third, when we compared with amount of circular mRNA made by these generators [BBa_K1332011] , [BBa_1859024], [BBa_K1859025] , [BBa_K1859026] .