Difference between revisions of "Part:BBa K1859024"
 
(5 intermediate revisions by 3 users not shown)
Line 2: Line 2:
  
 
<p>
 
<p>
−
mRNA circularize efficiently generator (outside complementarity)
+
mRNA circularize efficiently generator (inside complementarityⅠ)
 
</p>
 
</p>
  
 
<p>
 
<p>
−
His-RFPのmRNAを環状化させる効率を上げるために、私たちはこのジェネレーター[K1859024]をデザインした。
+
We designed this generator [BBa_K1859024] to improve circular efficiency of mRNA including His-RFP sequence.
 
</p>
 
</p>
  
Line 15: Line 15:
 
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859004" > [BBa_K1859004] </a>
 
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859004" > [BBa_K1859004] </a>
 
), His-RFP without stop codon
 
), His-RFP without stop codon
−
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_BBa_K1332002" >[BBa_K1332002] </a>
+
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332002" >[BBa_K1332002] </a>
 
, Lacl
 
, Lacl
 
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_R0010] </a>
 
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_R0010] </a>
 
, RBS
 
, RBS
−
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0034" >[BBa_B0034] </a>
+
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034" >[BBa_B0034] </a>
 
  and DT
 
  and DT
−
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_B0015] </a>
+
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_B0015" >[BBa_B0015] </a>
 
.
 
.
 
</p>
 
</p>
  
 
<p>
 
<p>
−
BBa_K1859003とBBa_K1859004には、タンパク質のフォールディングを整えるために設計したリンカーが入っている。
+
Linkers designed to adjust folding of protein are in
−
これらのリンカーのRNA配列は環状RNAの内部で相補的に結合する。
+
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859003" > [BBa_K1859003] </a>
 +
and
 +
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859004" > [BBa_K1859004] </a>
 +
.
 +
RNA sequences of these linkers bind complementary with each other in circular mRNA.
 
</p>
 
</p>
  
 
<p>
 
<p>
−
We found that circular efficiency of [BBa_1859026] was the best, when we compared with amount of circular mRNA made by these generators (BBa_1332011), (BBa_1859024), (BBa_18559025), (BBa_1859026).
+
We found that circular efficiency of [BBa_K1859024] was the third, when we compared with amount of circular mRNA made by these generators  
−
[BBa_1859024]は3番目に環状化の効率が良かった。
+
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332011" > [BBa_K1332011] </a>
 +
, [BBa_1859024],  
 +
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859025" > [BBa_K1859025] </a>
 +
,
 +
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859026" > [BBa_K1859026] </a>
 +
.
 
</p>
 
</p>
 
</html>
 
</html>

Latest revision as of 09:41, 22 September 2015

mRNA circularize efficiently generator (inside complementarityⅠ)

We designed this generator [BBa_K1859024] to improve circular efficiency of mRNA including His-RFP sequence.

The generator is comprised of Circular parts combined the linker( [BBa_K1859003] and [BBa_K1859004] ), His-RFP without stop codon [BBa_K1332002] , Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] .

Linkers designed to adjust folding of protein are in [BBa_K1859003] and [BBa_K1859004] . RNA sequences of these linkers bind complementary with each other in circular mRNA.

We found that circular efficiency of [BBa_K1859024] was the third, when we compared with amount of circular mRNA made by these generators [BBa_K1332011] , [BBa_1859024], [BBa_K1859025] , [BBa_K1859026] .