Difference between revisions of "Part:BBa K1859024"

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mRNA circularize efficiently generator (outside complementarity)
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mRNA circularize efficiently generator (inside complementarityⅠ)
 
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His-RFPの配列を含むmRNAを環状化させる効率を上げるために、私たちはこのジェネレーター[K1859024]をデザインした。
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We designed this generator [BBa_K1859024] to improve circular efficiency of mRNA including His-RFP sequence.
 
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BBa_K1859003とBBa_K1859004には、タンパク質のフォールディングを整えるために設計したリンカーが入っている。
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The generator is comprised of Circular parts combined the linker(
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このリンカーのRNA配列は環状RNAの内部で相補的に結合する。
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859003" > [BBa_K1859003] </a>
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The generator consists of 相補的になるように改良したCircular parts (
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859015" > [BBa_K1859015] </a>
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  and  
 
  and  
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859016" > [BBa_K1859016] </a>
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859004" > [BBa_K1859004] </a>
 
), His-RFP without stop codon
 
), His-RFP without stop codon
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_BBa_K1332002" >[BBa_K1332002] </a>
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332002" >[BBa_K1332002] </a>
 
, Lacl
 
, Lacl
 
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_R0010] </a>
 
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_R0010] </a>
 
, RBS
 
, RBS
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0034" >[BBa_B0034] </a>
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034" >[BBa_B0034] </a>
 
  and DT
 
  and DT
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_B0015] </a>
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_B0015" >[BBa_B0015] </a>
 
.
 
.
 
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このジェネレーター[BBa_1859026]で作成したRNAは環状mRNAの外側が相補的に結合するように設計されている。
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Linkers designed to adjust folding of protein are in
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859003" > [BBa_K1859003] </a>
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and
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859004" > [BBa_K1859004] </a>
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.
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RNA sequences of these linkers bind complementary with each other in circular mRNA.
 
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iGEM2014のジェネレーター[BBa_1332011]で作成した環状mRNAの量、[BBa_1859026]で作成した環状mRNAの量、[BBa_1859027]で作成した環状mRNAの量、[BBa_1859028]で作成した環状mRNAの量を比較すると、環状化の効率は[BBa_1859026]が最も良かった。
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We found that circular efficiency of [BBa_K1859024] was the third, when we compared with amount of circular mRNA made by these generators
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332011" > [BBa_K1332011] </a>
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, [BBa_1859024],
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859025" > [BBa_K1859025] </a>
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,
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859026" > [BBa_K1859026] </a>
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.
 
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Latest revision as of 09:41, 22 September 2015

mRNA circularize efficiently generator (inside complementarityⅠ)

We designed this generator [BBa_K1859024] to improve circular efficiency of mRNA including His-RFP sequence.

The generator is comprised of Circular parts combined the linker( [BBa_K1859003] and [BBa_K1859004] ), His-RFP without stop codon [BBa_K1332002] , Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] .

Linkers designed to adjust folding of protein are in [BBa_K1859003] and [BBa_K1859004] . RNA sequences of these linkers bind complementary with each other in circular mRNA.

We found that circular efficiency of [BBa_K1859024] was the third, when we compared with amount of circular mRNA made by these generators [BBa_K1332011] , [BBa_1859024], [BBa_K1859025] , [BBa_K1859026] .