Difference between revisions of "Part:BBa K1859029"
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| − | We designed this generator[ | + | We designed this generator[BBa_K1859029] to express long-chain protein with keeping functionality of the protein, giving the red fluorescence. |
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| − | The generator | + | The generator is comprised of Circular parts combined the linker( |
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859003" > [BBa_K1859003] </a> | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859003" > [BBa_K1859003] </a> | ||
and | and | ||
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859006" > [BBa_K1859006] </a> | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859006" > [BBa_K1859006] </a> | ||
), His-RFP without stop codon | ), His-RFP without stop codon | ||
| − | <a href= "https://parts.igem.org/wiki/index.php?title=Part: | + | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332002" >[BBa_K1332002] </a> |
, Lacl | , Lacl | ||
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_R0010] </a> | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_R0010] </a> | ||
, RBS | , RBS | ||
| − | <a href= "https://parts.igem.org/wiki/index.php?title=Part: | + | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034" >[BBa_B0034] </a> |
and DT | and DT | ||
| − | <a href= "https://parts.igem.org/wiki/index.php?title=Part: | + | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_B0015" >[BBa_B0015] </a> |
. | . | ||
</p> | </p> | ||
Latest revision as of 09:38, 22 September 2015
Histidine tag and RFP linker [HHHHHH] semi-permanent generator
We designed this generator[BBa_K1859029] to express long-chain protein with keeping functionality of the protein, giving the red fluorescence.
In the study of iGEM Gifu 2014, we made long-chain protein from Circular mRNA, but it lost functionality. It is thought that folding of the protein failed for a cause．
Therefore we designed some linkers to fix the folding of the protein. Then, we combined circular parts, the 3'side of the intron [BBa_K1332005] and the 5'side of the intron [BBa_K1332003] , with those linkers and made them parts. The generator is comprised of Circular parts combined the linker( [BBa_K1859003] and [BBa_K1859006] ), His-RFP without stop codon [BBa_K1332002] , Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] .
E. coli introduced the generator synthesized long-chain protein,but the protein had no fluorescence.