Difference between revisions of "Part:BBa K1859020:Experience"
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<img src="https://static.igem.org/mediawiki/2015/a/ab/Gifu-sds-dye.jpg" width="" height="240" vspace="10" hspace="50" align=""></img> | <img src="https://static.igem.org/mediawiki/2015/a/ab/Gifu-sds-dye.jpg" width="" height="240" vspace="10" hspace="50" align=""></img> | ||
<img src="https://static.igem.org/mediawiki/2015/6/60/Gifu-sds-page-combine.png" width="" height="240" vspace="10" hspace="50" align=""></img> | <img src="https://static.igem.org/mediawiki/2015/6/60/Gifu-sds-page-combine.png" width="" height="240" vspace="10" hspace="50" align=""></img> | ||
| − | <br>left: SDS-PAGE before dye center: SDS-PAGE after dye right: overlay | + | <br>left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay |
<br><br> | <br><br> | ||
There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.<br>. | There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.<br>. | ||
Revision as of 09:15, 22 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
===Applications of BBa_K1859020===
We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane E shows this sample.
left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay
There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.
.
User Reviews
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