Difference between revisions of "Part:BBa K1859020:Experience"

Revision as of 08:45, 22 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

===Applications of BBa_K1859020=== We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane E shows this sample.

There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.
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UNIQ72de5ad9dd46f8cb-partinfo-00000001-QINU UNIQ72de5ad9dd46f8cb-partinfo-00000002-QINU

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 <img src="https://static.igem.org/mediawiki/2015/0/04/Gifu-sds-before.jpeg" width="111" height="160" vspace="" hspace="" align="left"></img>
-<img src="https://static.igem.org/mediawiki/2015/a/ab/Gifu-sds-dye.jpg" width="146" height="160" vspace="10" hspace="50" align="right"></img>
+<img src="https://static.igem.org/mediawiki/2015/a/ab/Gifu-sds-dye.jpg" width="146" height="160" vspace="10" hspace="50" align=""></img>
+<img src="https://static.igem.org/mediawiki/2015/6/60/Gifu-sds-page-combine.png" width="" height="160" vspace="10" hspace="50" align=""></img>
+<br>
+There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.<br>.
-<b>Fluorescence of protein before dying by CBB</b>
-Fig.5 after dying by CBB<br><br></b></p>
-<img src="https://static.igem.org/mediawiki/2015/6/60/Gifu-sds-page-combine.png" width="" height="160" vspace="10" hspace="50" align="left"></img>
-<b>Fig.6 Overlapping Fig.4 and Fig.5 </b><br>
-&nbsp;&nbsp;There was not fluorescence at the place of long chain protein. We found that long chain protein didn’t have a function.
+</html>