Difference between revisions of "Part:BBa K1859020:Experience"

Revision as of 08:39, 22 September 2015

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Applications of BBa_K1859020

We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.

Lane E shows this sample.

Fluorescence of protein before dying by CBB Fig.5 after dying by CBB

</b></p> <img src="" width="" height="160" vspace="10" hspace="50" align="left"></img>

Fig.6 Overlapping Fig.4 and Fig.5

There was not fluorescence at the place of long chain protein. We found that long chain protein didn’t have a function.

User Reviews

UNIQe8f3a3c97838e5aa-partinfo-00000000-QINU UNIQe8f3a3c97838e5aa-partinfo-00000001-QINU

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 <img src="https://static.igem.org/mediawiki/2015/a/ab/Gifu-sds-dye.jpg" width="146" height="160" vspace="10" hspace="50" align="right"></img>
-<b>Fluorescence of protein before dying by CBB</b>  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<b>Fluorescence of protein before dying by CBB</b>
 Fig.5 after dying by CBB<br><br></b></p>
 <img src="https://static.igem.org/mediawiki/2015/6/60/Gifu-sds-page-combine.png" width="" height="160" vspace="10" hspace="50" align="left"></img>