Difference between revisions of "Part:BBa K1692023"

Revision as of 06:18, 22 September 2015

Ptet + Luxl

Overview

Our goal was to create an autolysis sytem that can lyse cell after cell growth reaches a threshold density. To do so, we incorporated two previous BioBricks ptet (BBa_R0040) and luxl generator (BBa_K805016). We wanted luxl to be produced constantly; however, it should not be overexpressed so we use a moderate strength promoter, ptet. We sequenced the construct and showed that it matched the intended construct. Using ptet-luxl generator, we were successful in designing our autolysis system (BBa_K1692024).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 717
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <h2> Overview </h2>
-Our goal was to create an autolysis sytem that can lyse cell after cell growth reaches a threshold density. To do so, we incorporated two previous BioBricks ptet ([https://parts.igem.org/Part:BBa_R0040 BBa_R0040]) and luxl generator ([https://parts.igem.org/Part:BBa_K805016 BBa_K805016]). We wanted luxl to be produced constantly; however, it should not be overexpressed so we use a moderate strength promoter, ptet. We sequenced the construct and showed that it matched the intended construct. Using ptet-luxl generator, we were successful in designing our autolysis system ([https://parts.igem.org/Part: BBa_K1692024 BBa_K1692024]).
+Our goal was to create an autolysis sytem that can lyse cell after cell growth reaches a threshold density. To do so, we incorporated two previous BioBricks ptet ([https://parts.igem.org/Part:BBa_R0040 BBa_R0040]) and luxl generator ([https://parts.igem.org/Part:BBa_K805016 BBa_K805016]). We wanted luxl to be produced constantly; however, it should not be overexpressed so we use a moderate strength promoter, ptet. We sequenced the construct and showed that it matched the intended construct. Using ptet-luxl generator, we were successful in designing our autolysis system ([https://parts.igem.org/Part:BBa_K1692024 BBa_K1692024]).
 <!-- Add more about the biology of this part here