Difference between revisions of "Part:BBa K1692023"
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| − | Our goal was to create an autolysis sytem that can lyse cell after cell growth reaches a threshold density. To do so, we incorporated two previous BioBricks ptet ([https://parts.igem.org/Part:BBa_R0040 BBa_R0040]) and luxl generator ([https://parts.igem.org/Part:BBa_K805016 BBa_K805016]). We wanted luxl to be produced constantly; however, it should not be overexpressed so we use a moderate strength promoter, ptet. We sequenced the construct and showed that it matched the intended construct. | + | Our goal was to create an autolysis sytem that can lyse cell after cell growth reaches a threshold density. To do so, we incorporated two previous BioBricks ptet ([https://parts.igem.org/Part:BBa_R0040 BBa_R0040]) and luxl generator ([https://parts.igem.org/Part:BBa_K805016 BBa_K805016]). We wanted luxl to be produced constantly; however, it should not be overexpressed so we use a moderate strength promoter, ptet. We sequenced the construct and showed that it matched the intended construct. Using ptet-luxl generator, we were successful in designing our autolysis system ([https://parts.igem.org/Part: BBa_K1692024 BBa_K1692024]). |
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Revision as of 06:17, 22 September 2015
Ptet + Luxl
Overview
Our goal was to create an autolysis sytem that can lyse cell after cell growth reaches a threshold density. To do so, we incorporated two previous BioBricks ptet (BBa_R0040) and luxl generator (BBa_K805016). We wanted luxl to be produced constantly; however, it should not be overexpressed so we use a moderate strength promoter, ptet. We sequenced the construct and showed that it matched the intended construct. Using ptet-luxl generator, we were successful in designing our autolysis system (BBa_K1692024 BBa_K1692024).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 717
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]