Difference between revisions of "Part:BBa K1692020"
 
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<partinfo>BBa_K1692020 short</partinfo>
 
<partinfo>BBa_K1692020 short</partinfo>
  
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This basic part is type II pantothenate kinase (panK) from <i>Staphylococcus aureus</i>, which means that it is not sensitive to feedback inhibition from coenzyme A or acetyl-CoA. This means that high levels of coenzyme A accumulate. We did not experiment with this gene alone; instead, we incorporated it into an already-existing biobrick (BBa_K1149051) to increase the production of poly-3-hydroxybuterate, a plastic of which coenzyme A is a precursor.  
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This basic part is a type II pantothenate kinase (panK) from <i>Staphylococcus aureus</i>. Pantothenate kinase is the enzyme that catalyzes the first step in the conversion from pantothenic acid to coenzyme A, and it controls the rate of the pathway because most bacterial panKs experience feedback from coenzyme A or its thioesters [1]. However, this panK is not sensitive to feedback inhibition from coenzyme A or acetyl-CoA, meaning that high levels of coenzyme A accumulate [2]. We did not experiment with this gene alone; instead, we incorporated it into an already-existing BioBrick ([https://parts.igem.org/Part:BBa_K1149051 BBa_K1149051]) to increase the production of poly-3-hydroxybuterate, a plastic of which coenzyme A is a precursor. Our composite part ([https://parts.igem.org/Part:BBa_K1692021 BBa_K1692021]) can explain more about our success with using Pank to increase synthesis of PHA.
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References:
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[1] Leonardi, R. et al. Coenzyme A: Back in action. Progress in Lipid Research 44, 2005. 125 – 153.
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[2] Leonardi, R. et al. A Pantothenate Kinase from Staphylococcus aureus Refractory to Feedback Regulation by Coenzyme A. The Journal of Biological Chemistry 280, 2005. 3312 – 3322.
  
 
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<!-- Add more about the biology of this part here

Latest revision as of 05:47, 22 September 2015

S. aureus type II PanK (CoaA)

This basic part is a type II pantothenate kinase (panK) from Staphylococcus aureus. Pantothenate kinase is the enzyme that catalyzes the first step in the conversion from pantothenic acid to coenzyme A, and it controls the rate of the pathway because most bacterial panKs experience feedback from coenzyme A or its thioesters [1]. However, this panK is not sensitive to feedback inhibition from coenzyme A or acetyl-CoA, meaning that high levels of coenzyme A accumulate [2]. We did not experiment with this gene alone; instead, we incorporated it into an already-existing BioBrick (BBa_K1149051) to increase the production of poly-3-hydroxybuterate, a plastic of which coenzyme A is a precursor. Our composite part (BBa_K1692021) can explain more about our success with using Pank to increase synthesis of PHA.


References:

[1] Leonardi, R. et al. Coenzyme A: Back in action. Progress in Lipid Research 44, 2005. 125 – 153.

[2] Leonardi, R. et al. A Pantothenate Kinase from Staphylococcus aureus Refractory to Feedback Regulation by Coenzyme A. The Journal of Biological Chemistry 280, 2005. 3312 – 3322.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 10
    Illegal NheI site found at 33
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 420
  • 1000
    COMPATIBLE WITH RFC[1000]