Difference between revisions of "Part:BBa K1582024"
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<partinfo>BBa_K1582024 parameters</partinfo>
 
<partinfo>BBa_K1582024 parameters</partinfo>
 
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===Usage===
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This fusion protein is designed to degrade PET into terephthalic acid and ethylene glycol more powerfully. Compared to single Thc_Cut1, the rate of PET emzymolysis is supposed to be improved by Thc_Cut1-Janus fusion.
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===Biology===
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Thc_Cut1 is a kind of cutinase, the details of which can be seen at BBa_K1582028. Class II hydrophobins sJanus are small secreted fungal proteins which can be found in filamentous fungi, which play a role in a broad range of processes in the growth and development of filamentous fungi. Their assembly shows thread-like structure. They could be expressed in prokaryotic cells like E.coli. <br>
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According to some research, when Janus is added to the reaction of cutinase and plastics, the reaction rate is supposed to have an obvious improvement.<br>
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Based on the above background, we design to use the method of fusion protein to combine cutinase and Janus compactly, through the establishment of fusion protein, we can found if we can further promote the stimulation rate of the plastics degradation. Meanwhile, from the data about fusion protein, we can try to uncover the functional mechanism of the stimulation to the plastics degradation which is unknown until now. <br>

Revision as of 04:36, 22 September 2015

Thc_Cut1+sJanus Fusion Protein


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1048
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1057


Usage

This fusion protein is designed to degrade PET into terephthalic acid and ethylene glycol more powerfully. Compared to single Thc_Cut1, the rate of PET emzymolysis is supposed to be improved by Thc_Cut1-Janus fusion.

Biology

Thc_Cut1 is a kind of cutinase, the details of which can be seen at BBa_K1582028. Class II hydrophobins sJanus are small secreted fungal proteins which can be found in filamentous fungi, which play a role in a broad range of processes in the growth and development of filamentous fungi. Their assembly shows thread-like structure. They could be expressed in prokaryotic cells like E.coli.
According to some research, when Janus is added to the reaction of cutinase and plastics, the reaction rate is supposed to have an obvious improvement.
Based on the above background, we design to use the method of fusion protein to combine cutinase and Janus compactly, through the establishment of fusion protein, we can found if we can further promote the stimulation rate of the plastics degradation. Meanwhile, from the data about fusion protein, we can try to uncover the functional mechanism of the stimulation to the plastics degradation which is unknown until now.