Difference between revisions of "Part:BBa K1615060"

 
 
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<partinfo>BBa_K1615060 short</partinfo>
 
<partinfo>BBa_K1615060 short</partinfo>
  
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Heroin Esterase fused N terminally to dCBD + N terminal linker driven by the IPTG inducible PLlac 0-1 promoter.
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Heroin esterase fused to the N terminal of dCBD + N terminal linker (BBa_K1615050) driven by the IPTG inducible PLlac 0-1 promoter (BBa_K314103). This allows the heroin esterase CBD fusion to express when induced with IPTG.
  
 
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Latest revision as of 00:03, 22 September 2015

HerE fused to Linker+dCBD driven by LacI promoter

Heroin esterase fused to the N terminal of dCBD + N terminal linker (BBa_K1615050) driven by the IPTG inducible PLlac 0-1 promoter (BBa_K314103). This allows the heroin esterase CBD fusion to express when induced with IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1674
    Illegal BamHI site found at 2430
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
    Illegal NgoMIV site found at 1648
  • 1000
    COMPATIBLE WITH RFC[1000]