Difference between revisions of "Part:BBa K1639009"
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===Usage and Biology=== | ===Usage and Biology=== | ||
In our project Cancer module consist of three parts. First one produces tTA to activate expression of mLacI protein form pTRE. "m" in mLacI stands for mammalian, it's optimized version of bacterial LacI for expression in eucaryots.Produced mLacI would suppress CMV IE promoter through lac operators.''(Figure 1)'' | In our project Cancer module consist of three parts. First one produces tTA to activate expression of mLacI protein form pTRE. "m" in mLacI stands for mammalian, it's optimized version of bacterial LacI for expression in eucaryots.Produced mLacI would suppress CMV IE promoter through lac operators.''(Figure 1)'' | ||
| − | [[File: | + | [[File:ATOMS-Turkiye_cancer_switch_1.1.png|center|500px|thumb|'''Figure 1:'''A detailed diagram of miRNA recognition system]] |
This part composed of two lac operators which followed up with DsRed protein (red flourescent protein derived from ''Discosoma spp'') and miRNA binding sites at 3'end. This miRNA binding sites are complementary to low-miRNA in gastric cancer cells. In normal cells the level of these miRNas are higher than in gastric cancerous cells they would suppress expression of DsRed protein. | This part composed of two lac operators which followed up with DsRed protein (red flourescent protein derived from ''Discosoma spp'') and miRNA binding sites at 3'end. This miRNA binding sites are complementary to low-miRNA in gastric cancer cells. In normal cells the level of these miRNas are higher than in gastric cancerous cells they would suppress expression of DsRed protein. | ||
Revision as of 17:35, 21 September 2015
LacI regulated DsRed with miR 26a and miR 375 binding sites
Usage and Biology
In our project Cancer module consist of three parts. First one produces tTA to activate expression of mLacI protein form pTRE. "m" in mLacI stands for mammalian, it's optimized version of bacterial LacI for expression in eucaryots.Produced mLacI would suppress CMV IE promoter through lac operators.(Figure 1)
This part composed of two lac operators which followed up with DsRed protein (red flourescent protein derived from Discosoma spp) and miRNA binding sites at 3'end. This miRNA binding sites are complementary to low-miRNA in gastric cancer cells. In normal cells the level of these miRNas are higher than in gastric cancerous cells they would suppress expression of DsRed protein.
Cloning and Expression
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 835
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 593
Illegal BamHI site found at 876 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 535
- 1000COMPATIBLE WITH RFC[1000]