Difference between revisions of "Part:BBa K1616013"
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===Design Notes===
 
===Design Notes===
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Illegal sites have been checked.  
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All BioBrick parts used for assembling the composite part are compatible with the RFC10 Biobrick standard.
  
  

Revision as of 17:15, 21 September 2015

ccdB reversed - RBS and double T7 terminator

ccdB comes from the ccd system in Escherichia coli F plasmid and acts as a gyrase poison. The CcdB protein, constitutively expressed by P1010, is lethal to most of the BioBrick cell strains, only DB3.1 is resistant.


Usage and Biology

This part can be used with a reversed promoter to induce cell lysis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 226


Design Notes

All BioBrick parts used for assembling the composite part are compatible with the RFC10 Biobrick standard.


Source

This part is composed of ccdB reversed, RBS reversed and the double terminator T7 reversed.