Difference between revisions of "Part:BBa K1323014:Experience"
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<I>alicia.dubinski</I>
 
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The OD600 graph shows that all of the strains grew in the 96-well plate. The GFP fluorescence intensity graph shows that the J23101 promoter effectively expressed the GFP protein while the strain with no promoter did not have GFP expression. When the xylose inducible promoter was in just LB media, it did not express GFP. When xylose was added, the promoter also did not express GFP. This means that the mutation made to remove the illegal site also affected the promoter. The mutated promoter does not work, even when it is induced with 2% xylose.  
 
The OD600 graph shows that all of the strains grew in the 96-well plate. The GFP fluorescence intensity graph shows that the J23101 promoter effectively expressed the GFP protein while the strain with no promoter did not have GFP expression. When the xylose inducible promoter was in just LB media, it did not express GFP. When xylose was added, the promoter also did not express GFP. This means that the mutation made to remove the illegal site also affected the promoter. The mutated promoter does not work, even when it is induced with 2% xylose.  
 
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Revision as of 16:49, 21 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1323014

User Reviews

UNIQ13dea3631c868d0d-partinfo-00000000-QINU UNIQ13dea3631c868d0d-partinfo-00000001-QINU