Difference between revisions of "Part:BBa K1639024"

(Usage and Biology)
(Usage and Biology)
Line 8: Line 8:
  
 
This part is same with BBa_K1639000 but without GFP reporter protein
 
This part is same with BBa_K1639000 but without GFP reporter protein
[[File:toehold nsapgene.png|center|500px|thumb|Schematic diagram of Toehold-RNA device]]
+
[[File:toehold nsapgene.png|center|700px|thumb|Schematic diagram of Toehold-RNA device]]
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 14:44, 21 September 2015

Toehold riboregulator without reporter

This part is composed of approximately 100 nt RNA molecule. It's 30 nt is completely complementary to BBa_K1639001. Together they constitute AND logic gate part of our project. It's a new and flexible approach to AND gate switches because of sequence variablity and ease of modification.


Usage and Biology

This part is same with BBa_K1639000 but without GFP reporter protein

Schematic diagram of Toehold-RNA device

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 117
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]