Difference between revisions of "Part:BBa K1639007:Design"
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This part contains an additional BamHI restriction site at beginning of DAMP-Pex sequence and a XhoI restriction site between DAMP-Pex and double terminator(B0010-B0012). Also have a TEV Cleaveage site(ENLYFQS) between DAMP and Pex. | This part contains an additional BamHI restriction site at beginning of DAMP-Pex sequence and a XhoI restriction site between DAMP-Pex and double terminator(B0010-B0012). Also have a TEV Cleaveage site(ENLYFQS) between DAMP and Pex. | ||
| − | + | To achieve constitutive expression of this protein complex we put lacIq promoter at the beginning. It is a single base changed version of ''lacI'' promoter which boosts expression of gene about 10-fold. As this promoter cannot be terminated by T7 terminator like most of our parts we inserted double terminator (B0010-B0012) at the end. | |
===Source=== | ===Source=== | ||
Latest revision as of 13:00, 21 September 2015
DAMP-Pexiganan
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 108
Illegal XhoI site found at 508 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part contains an additional BamHI restriction site at beginning of DAMP-Pex sequence and a XhoI restriction site between DAMP-Pex and double terminator(B0010-B0012). Also have a TEV Cleaveage site(ENLYFQS) between DAMP and Pex.
To achieve constitutive expression of this protein complex we put lacIq promoter at the beginning. It is a single base changed version of lacI promoter which boosts expression of gene about 10-fold. As this promoter cannot be terminated by T7 terminator like most of our parts we inserted double terminator (B0010-B0012) at the end.
Source
none. Synthetic.