Difference between revisions of "Part:BBa K1618027"

Revision as of 12:14, 21 September 2015

GlgB-Rv3037c with IPTG-inducible promoter

K1618024 contains the coding sequences for GlgB from E. coli and the coding sequence for Rv3034 from Mycobacterium tuberculosis (strain ATCC 25618) under control of the LacI promoter. GlgB cleaves alpha-1,4 linkages in glycogen chains and transfers the cleaved branch onto the main glycogen chain using an alpha-1,6 linkage to make a branch point. Rv3034c is a putative S-adenosylmethionine-dependent methyltransferase. This part will potentially make branched, modified glycogen.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 283
Illegal BamHI site found at 1491
Illegal BamHI site found at 3142
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2952
Illegal NgoMIV site found at 3125
Illegal NgoMIV site found at 3449
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 917

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 <partinfo>BBa_K1618027 short</partinfo>
-Rv3037c is a genetic sequence which encodes for a putative acyltransferase enzyme which will hopefully acylate glycogen. This is preceded by GlgB which encodes for a glycogen branching enzyme which cleaves alpha-1,4 linkages in glycogen and re-anneals the cleaved strand onto the main glycogen strand to create an alpha-1,6 linkage to create a branch. Promoted by an IPTG-inducible promoter and encoded within the standard pSB1C3 vector. The bacteria are therefore chloramphenicol resistant.
+K1618024 contains the coding sequences for GlgB from E. coli and the coding sequence for Rv3034 from Mycobacterium tuberculosis (strain ATCC 25618) under control of the LacI promoter. GlgB cleaves alpha-1,4 linkages in glycogen chains and transfers the cleaved branch onto the main glycogen chain using an alpha-1,6 linkage to make a branch point. Rv3034c is a putative S-adenosylmethionine-dependent methyltransferase.  This part will potentially make branched, modified glycogen.
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