Difference between revisions of "Part:BBa K1678003"
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<partinfo>BBa_K1678003 short</partinfo> | <partinfo>BBa_K1678003 short</partinfo> | ||
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Coding sequence has been codon optimized for Lactobacillus plantarum. | Coding sequence has been codon optimized for Lactobacillus plantarum. | ||
| − | Protein is expressed by a L.plantarum synthetic promoter p48 BBa_K1678004. | + | Protein is expressed by a L.plantarum synthetic promoter p48 <a href="https://parts.igem.org/Part:BBa_K1678004:Experience">BBa_K1678004</a>. |
The gene is terminated by the Tldh terminator (Spath et al. Microbial Cell Factories 2012, 11:141). | The gene is terminated by the Tldh terminator (Spath et al. Microbial Cell Factories 2012, 11:141). | ||
Latest revision as of 11:58, 21 September 2015
RibT promoted by p48 synthetic promoter
Bacillus subtilis RibT gene for riboflavin production. RibT code for the Beta-chain of the riboflavin synthase.
Coding sequence has been codon optimized for Lactobacillus plantarum.
Protein is expressed by a L.plantarum synthetic promoter p48 <a href="https://parts.igem.org/Part:BBa_K1678004:Experience">BBa_K1678004</a>.
The gene is terminated by the Tldh terminator (Spath et al. Microbial Cell Factories 2012, 11:141).
The RBS and the spacing between the RBS and the coding sequence are optimized for Lactobacillus plantarum (Tauer et al. Microbial Cell Factories 2014, 13:150).
The expressed protein catalyses one step in the riboflavin pathway.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 21
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 552