Difference between revisions of "Part:BBa K1678006:Design"

 
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===Design Notes===
 
===Design Notes===
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None
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The promoter comes from the following publication: 'A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum', Microbiology, 2006.<br>
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+
The T<sub>ldh</sub> terminator comes from L. buchneri lactate dehydrogenase gene. (also used in :'Spath et al. Microbial Cell Factories 2012, 11:141').<br>
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+
The RBS comes from the following publication: 'Tauer et al. Microbial Cell Factories 2014, 13:150'.<br>
 +
The whole cassette was artificially synthesized.
  
 
===Source===
 
===Source===
−
 
−
The promoter comes from the following publication: 'A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum', Microbiology, 2006
 
−
The terminator comes from the following publication: 'Spath et al. Microbial Cell Factories 2012, 11:141'
 
−
The RBS comes from the following publication: 'Tauer et al. Microbial Cell Factories 2014, 13:150'
 
−
The whole cassette was artificially synthesized.
 
  
 
===References===
 
===References===

Latest revision as of 11:54, 21 September 2015


mCerulean gene cassette with P48 promoter and TldH terminator for lactobacillus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 7
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 883


Design Notes

The promoter comes from the following publication: 'A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum', Microbiology, 2006.
The Tldh terminator comes from L. buchneri lactate dehydrogenase gene. (also used in :'Spath et al. Microbial Cell Factories 2012, 11:141').
The RBS comes from the following publication: 'Tauer et al. Microbial Cell Factories 2014, 13:150'.
The whole cassette was artificially synthesized.

Source

References