Difference between revisions of "Part:BBa K1614001"
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Therefore we used this ribozyme for all BBF RFC 110 parts, as the 3' limiter. Thereby the production of RNA product with the user-defined ends is ensured. For more details have a look at our cloning and transcription cassette <br/> | Therefore we used this ribozyme for all BBF RFC 110 parts, as the 3' limiter. Thereby the production of RNA product with the user-defined ends is ensured. For more details have a look at our cloning and transcription cassette <br/> | ||
| − | [[File:HDV_Cleavage_Behavior.png|left|600px|thumb|'''Fig.1: PAGE analysis of HDV cleavage behavior.''' The RNA of interest appears as a defined band under UV-shadowing. Higher yields for the desired product with high fidelity can be obtained by the PCR extraction of the [[Part:BBa_K1614002|transcription cassette]] with [[Part:BBa_K1614005 BBa_K1614005]] [[Part:BBa_K1614006|BBa_K1614006]]. Uncleaved product can be observed as the third band.]] | + | [[File:HDV_Cleavage_Behavior.png|left|600px|thumb|'''Fig.1: PAGE analysis of HDV cleavage behavior.''' The RNA of interest appears as a defined band under UV-shadowing. Higher yields for the desired product with high fidelity can be obtained by the PCR extraction of the [[Part:BBa_K1614002|transcription cassette]] with [[Part:BBa_K1614005 |BBa_K1614005]] [[Part:BBa_K1614006|BBa_K1614006]]. Uncleaved product can be observed as the third band.]]<br/> |
Revision as of 07:23, 21 September 2015
Hepatitis Delta Virus(HDV) Ribozyme
The hepatitis delta virus ribozyme is a self cleaving ribozyme originating from hepatitis delta virus. The cleavage occurs at the ribozymes 5' end independent from the upstream sequence. Therefore it is highly suitable to create defined ends for functional RNA expression. Furthermore its behvior is specific and shows good efficiency.
Therefore we used this ribozyme for all BBF RFC 110 parts, as the 3' limiter. Thereby the production of RNA product with the user-defined ends is ensured. For more details have a look at our cloning and transcription cassette
Fig.1: PAGE analysis of HDV cleavage behavior. The RNA of interest appears as a defined band under UV-shadowing. Higher yields for the desired product with high fidelity can be obtained by the PCR extraction of the transcription cassette with BBa_K1614005 BBa_K1614006. Uncleaved product can be observed as the third band.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2
Illegal NgoMIV site found at 31 - 1000COMPATIBLE WITH RFC[1000]