Difference between revisions of "Part:BBa K1725040"
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<partinfo>BBa_K1725040 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1725040 SequenceAndFeatures</partinfo> | ||
| + | PhlF gave 83-fold repression of GFP expression from PphlF (<bbpart>BBa_K1725000</bbpart>), whereas the control, TetR (<bbpart>BBa_C0040</bbpart>), gave only 33-fold repression of pL-tet (<bbpart>BBa_R0040</bbpart>). (figure 1) A lower expression level of PhlF completely represses GFP expression from PphlF, compared to the higher expression level of TetR required for pL-tet. (figure 2) | ||
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| + | https://static.igem.org/mediawiki/parts/7/78/Glasgow_2015_Fold_Repression_Graph.png | ||
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| + | <b>Figure 1 Fold Repression. BioBricks: K1725082 (pL-tet driving GFP expression), K1725031 (pL-lac driving TetR expression), K1725001 (PphlF driving GFP expression), and K1725042 (pL-lac driving PhlF expression). Repressor protein expression induced with 100μM IPTG. Mean and standard deviation of replicates were calculated to give value and error bars, and normalised against a negative control.</b> | ||
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| + | https://static.igem.org/mediawiki/parts/6/6e/Glasgow_2015_Varied_IPTG_Graph.png | ||
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| + | <b>Figure 2 Varied Repression Levels. BioBricks: K1725082 (pL-tet driving GFP expression), K1725031 (pL-lac driving TetR expression), K1725001 (PphlF driving GFP expression), and K1725042 (pL-lac driving PhlF expression). Repressor protein expression induced with 100μM, 30 μM, 10 μM, 3 μM, and 0 μM IPTG. Mean and standard deviation of replicates were calculated to give value and error bars, and normalised against a negative control.</b> | ||
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Latest revision as of 19:22, 20 September 2015
phlF encoding PhlF repressor from Pseudomonas protegens Pf-5
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
PhlF gave 83-fold repression of GFP expression from PphlF (BBa_K1725000), whereas the control, TetR (BBa_C0040), gave only 33-fold repression of pL-tet (BBa_R0040). (figure 1) A lower expression level of PhlF completely represses GFP expression from PphlF, compared to the higher expression level of TetR required for pL-tet. (figure 2)
Figure 1 Fold Repression. BioBricks: K1725082 (pL-tet driving GFP expression), K1725031 (pL-lac driving TetR expression), K1725001 (PphlF driving GFP expression), and K1725042 (pL-lac driving PhlF expression). Repressor protein expression induced with 100μM IPTG. Mean and standard deviation of replicates were calculated to give value and error bars, and normalised against a negative control.
Figure 2 Varied Repression Levels. BioBricks: K1725082 (pL-tet driving GFP expression), K1725031 (pL-lac driving TetR expression), K1725001 (PphlF driving GFP expression), and K1725042 (pL-lac driving PhlF expression). Repressor protein expression induced with 100μM, 30 μM, 10 μM, 3 μM, and 0 μM IPTG. Mean and standard deviation of replicates were calculated to give value and error bars, and normalised against a negative control.