Difference between revisions of "Part:BBa M1000"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_M1000 short</partinfo> | <partinfo>BBa_M1000 short</partinfo> | ||
− | Left end of a linear | + | *Left end of a linear transposome sequence containing the 19 bp mosaic end in the forward orientation. The sequence also contains a PvuII cut site which releases the transpososome when cut with PvuII. Constructed compound parts intended for transposition must not contain other PvuII cut sites, or be PCR-amplified with blunt-end-producing enzymes (Pfu, Phusion) at the PvuII cut site between CAG and CTG. |
− | This part, and the companion part M1001 provide | + | *This part, and the companion part M1001 provide BioBrick tools to construct Tn5 transposomes: linear DNA sequences which can be cut ''in vitro'' from a plasmid backbone, or created by PCR, combined ''in vitro'' with purified Tn5 transposase protein, electroportated into cells, and finally randomly insert into the bacterial chromosome. |
− | The parts assembled between the two mosaic ends can be delivered, including antibiotic resistance markers or other functional chromosomal insertions. | + | *The parts assembled between the two mosaic ends can be delivered, including antibiotic resistance markers or other functional chromosomal insertions. |
+ | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_M1000 SequenceAndFeatures</partinfo> | <partinfo>BBa_M1000 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_M1000 parameters</partinfo> | <partinfo>BBa_M1000 parameters</partinfo> | ||
+ | <!-- --> |
Latest revision as of 20:38, 12 April 2007
Left mosaic end for Tn5 transpososome
- Left end of a linear transposome sequence containing the 19 bp mosaic end in the forward orientation. The sequence also contains a PvuII cut site which releases the transpososome when cut with PvuII. Constructed compound parts intended for transposition must not contain other PvuII cut sites, or be PCR-amplified with blunt-end-producing enzymes (Pfu, Phusion) at the PvuII cut site between CAG and CTG.
- This part, and the companion part M1001 provide BioBrick tools to construct Tn5 transposomes: linear DNA sequences which can be cut in vitro from a plasmid backbone, or created by PCR, combined in vitro with purified Tn5 transposase protein, electroportated into cells, and finally randomly insert into the bacterial chromosome.
- The parts assembled between the two mosaic ends can be delivered, including antibiotic resistance markers or other functional chromosomal insertions.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]