Difference between revisions of "Part:BBa J31006"

Latest revision as of 19:47, 12 April 2007

tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]

This is the coding region for tetracyline resistance [TetA(C) inner-membrane-associated protein] in the reverse orientation. Please read the note about this part's BioBrick cloning sites on the part design page.

Usage and Biology

If RBS (reverse) and a promoter (reverse) are placed to the right of this part, the cell will express tetracycline resistance.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1043
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 897
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 343
Illegal NgoMIV site found at 503
Illegal NgoMIV site found at 871
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_J31006 short</partinfo>
-This is the coding region for Tetracyline resistance in the reverse orientation. If a promoter adn RBS are placed downstream and the entire region, from promoter to stop codon is flipped the cell will express tetracycline resistance. <font size='3'> <font color='red'> PLEASE READ THE NOTE ABOUT THIS PART'S BIOBRICKS ON THE PART DESIGN PAGE.</font size> </font color>
+This is the coding region for tetracyline resistance [TetA(C) inner-membrane-associated protein] in the reverse orientation. Please read the note about this part's BioBrick cloning sites on the part design page.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+If RBS (reverse) and a promoter (reverse) are placed to the right of this part, the cell will express tetracycline resistance.
-<!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_J31006 SequenceAndFeatures</partinfo>