Revision as of 18:12, 20 September 2015

Pcons+B0034+Lpp-OmpA-N+scFv(Anti-HER2)

Introduction:

Fig.1 Pcons+RBS+Lpp-OmpA-N+Anti-HER2

This year we want to provide a customized platform. We provide two libraries of Pcon+RBS+OmpA-scFv and Pcons+RBS+Fluorescence+Ter into E. coli. Therefore, our customers can choose any scfv and any fluorescence protein. Our team will then co-transform the two plasmids, which helps us tailor our product to the wishes of our customers.

Experiment

Fig.3 The PCR result of the Pcons+B0034+Lpp-OmpA-N+scFv. The DNA sequence length is around 1100~1300 bp, so the PCR products should appear at 1300~1500 bp.

After assemble the DNA sequences from the basic parts, we recombined each Pcons+RBS+Lpp-OmpA-N+scFv gene to PSB1C3 backbones and conducted a PCR experiment to check the size of each of the parts. The DNA sequence length of these parts is around 1100~1300 bp. In this PCR experiment, the PCR products size should be near at 1300~1500 bp. The Fig. 3 showed the correct size of this part, and proved that we successful ligated the sequence onto an ideal backbone.

Fig.4 Pcons+RBS+Lpp-OmpA-N+scFv(Anti-HER2)

Sequence and Features