Difference between revisions of "Part:BBa K1694025"
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<h1>'''Introduction:'''</h1>
 
<h1>'''Introduction:'''</h1>
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By ligating the constitutive promoter (BBa_J23101), strong ribosome binding site (BBa_B0034) and Lpp-OmpA-scFv, we were able to display scFv(Trastuzumab) outside the E.coli cell membrane.
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[[File:HERHALF.png|600px|thumb|center|'''Fig.1''' Pcons+RBS+Lpp-OmpA-N+Anti-HER2]]
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This year we want to provide a customized platform. We provide two libraries of Pcon+RBS+OmpA-scFv and Pcons+RBS+Fluorescence+Ter into E. coli. Therefore, our customers can choose any scfv and any fluorescence protein. Our team will then co-transform the two plasmids, which helps us tailor our product to the wishes of our customers.
 
This year we want to provide a customized platform. We provide two libraries of Pcon+RBS+OmpA-scFv and Pcons+RBS+Fluorescence+Ter into E. coli. Therefore, our customers can choose any scfv and any fluorescence protein. Our team will then co-transform the two plasmids, which helps us tailor our product to the wishes of our customers.
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<h1>'''Experiment'''</h1>
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[[File:PROHPCR.png|200px|thumb|left|'''Fig.3''' The PCR result of the Pcons+B0034+Lpp-OmpA-N+scFv. The DNA sequence length is around 1100~1300 bp, so the PCR products should appear at 1300~1500 bp.]]
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After assemble the DNA sequences from the basic parts, we recombined each Pcons+RBS+Lpp-OmpA-N+scFv gene to PSB1C3 backbones and conducted a PCR experiment to check the size of each of the parts. The DNA sequence length of these parts is around 1100~1300 bp. In this PCR experiment, the PCR products size should be near at 1300~1500 bp. The Fig. 3 showed the correct size of this part, and proved that we successful ligated the sequence onto an ideal backbone.
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[[File:PROH.png|200px|thumb|center|'''Fig.4''' Pcons+RBS+Lpp-OmpA-N+scFv(Anti-HER2)]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 18:10, 20 September 2015

Pcons+B0034+Lpp-OmpA-N+scFv(Anti-HER2)

Introduction:

Fig.1 Pcons+RBS+Lpp-OmpA-N+Anti-HER2



This year we want to provide a customized platform. We provide two libraries of Pcon+RBS+OmpA-scFv and Pcons+RBS+Fluorescence+Ter into E. coli. Therefore, our customers can choose any scfv and any fluorescence protein. Our team will then co-transform the two plasmids, which helps us tailor our product to the wishes of our customers.

Experiment


Fig.3 The PCR result of the Pcons+B0034+Lpp-OmpA-N+scFv. The DNA sequence length is around 1100~1300 bp, so the PCR products should appear at 1300~1500 bp.

After assemble the DNA sequences from the basic parts, we recombined each Pcons+RBS+Lpp-OmpA-N+scFv gene to PSB1C3 backbones and conducted a PCR experiment to check the size of each of the parts. The DNA sequence length of these parts is around 1100~1300 bp. In this PCR experiment, the PCR products size should be near at 1300~1500 bp. The Fig. 3 showed the correct size of this part, and proved that we successful ligated the sequence onto an ideal backbone.

Fig.4 Pcons+RBS+Lpp-OmpA-N+scFv(Anti-HER2)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 741
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 451
  • 1000
    COMPATIBLE WITH RFC[1000]