Difference between revisions of "Part:BBa K1806002:Experience"

 
(→‎Applications of BBa_K1806002)
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===Applications of BBa_K1806002===
 
===Applications of BBa_K1806002===
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== Cloning ==
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The system was ligated with the pSB1C3 cloning vector and then transformated to be verified with Colony PCR.
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The RFP present in the iGEM Registry contains the Hind3 enzyme restriction site unlike the RFP sequence present in this part. Hence, the part was cut with the EcoR1 and Hind3 restriction enzymes.
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Tagged in the gel runs below as G-Block 4, it can be seen that the gel runs yielded accurate base length.
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[[File:6002-1.png]] [[File:6002-2.png]]
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[[File:6002-3.png]] [[File:6002-4.png]]
  
 
===User Reviews===
 
===User Reviews===

Revision as of 16:10, 20 September 2015


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1806002

Cloning

The system was ligated with the pSB1C3 cloning vector and then transformated to be verified with Colony PCR. The RFP present in the iGEM Registry contains the Hind3 enzyme restriction site unlike the RFP sequence present in this part. Hence, the part was cut with the EcoR1 and Hind3 restriction enzymes.

Tagged in the gel runs below as G-Block 4, it can be seen that the gel runs yielded accurate base length.

6002-1.png 6002-2.png

6002-3.png 6002-4.png

User Reviews

UNIQbfc81d3f3f72bdc6-partinfo-00000000-QINU UNIQbfc81d3f3f72bdc6-partinfo-00000001-QINU