Difference between revisions of "Part:BBa I13602"
(→Usage and Biology) |
(→Usage and Biology) |
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The Ptet promoter used is a medium strength promoter. | The Ptet promoter used is a medium strength promoter. | ||
<p>'''Figure 1: Fluorescence microscopy of E. coli DH5alpha bearing I13602'''</p> | <p>'''Figure 1: Fluorescence microscopy of E. coli DH5alpha bearing I13602'''</p> | ||
| − | <p>'''(A)''' Differential interference contrast | + | <p>'''(A)''' Differential interference contrast; '''(B)''' excitation with led 437 nm/17nm (CFP), 200 ms of exposure</p> |
[[File:Paris_Saclay-I13602.png]] | [[File:Paris_Saclay-I13602.png]] | ||
Revision as of 13:01, 20 September 2015
Tet operator with CFP reporter (with LVA tag) [R/Tc+]
Usage and Biology
This part can be used to test the classical Tet repression system with a CFP reporter. The Ptet promoter used is a medium strength promoter.
Figure 1: Fluorescence microscopy of E. coli DH5alpha bearing I13602
(A) Differential interference contrast; (B) excitation with led 437 nm/17nm (CFP), 200 ms of exposure
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]