Difference between revisions of "Part:BBa K1486003"

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Flexible linker used to fuse two proteins.
 
Flexible linker used to fuse two proteins.
  
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===Applications of BBa_K1486003===
 
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'''Group''': iGEM15_Fudan<br>   
 
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'''Author''': Xuhang Li <br>       
 
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'''Summary''':We developed a new part(BBa_K1777005) based on this parts.When we planned to use this part to link our fusion component,we found it hard to design a proper primer to amplify it because of mutiple primer binding site as a result of tandem GGGS.Therefore, we first improved it by change the first GGGS to a natural linker obtained from linker database.In addition,the codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Such an improved GS linker could be easily assembled to your protein via overlap PCR and offer the property of nuclear localization.<br>
 
 
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K1486003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1486003 SequenceAndFeatures</partinfo>
  

Latest revision as of 12:25, 20 September 2015

Flexible linker 2x (GGGS)

Flexible linker used to fuse two proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]