Difference between revisions of "Part:BBa K1486003"
 
(5 intermediate revisions by 3 users not shown)
Line 5: Line 5:
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
−
<br>
 
−
Group: iGEM15_Fudan<br>
 
−
Author: Xuhang Li/n<br>
 
−
Summary:We developed a new part(BBa_K1777005) based on this parts. The codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR.
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K1486003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1486003 SequenceAndFeatures</partinfo>
  

Latest revision as of 12:25, 20 September 2015

Flexible linker 2x (GGGS)

Flexible linker used to fuse two proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]