Difference between revisions of "Part:BBa K1486003:Experience"
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'''Group''': iGEM15_Fudan<br> | '''Group''': iGEM15_Fudan<br> | ||
'''Author''': Xuhang Li <br> | '''Author''': Xuhang Li <br> | ||
| − | '''Summary''':We developed a new part(BBa_K1777005) based on this parts.When we planned to use this part to link our fusion component,we found it hard to design a proper primer to amplify it because of mutiple primer binding site as a result of tandem GGGS.Therefore, we first improved it by change the first GGGS to a natural linker obtained from linker database.In addition,the codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Such an improved GS linker could be easily assembled to your protein via overlap PCR and offer the property of nuclear localization. | + | '''Summary''':We developed a new part([http://https://parts.igem.org/Part:BBa_K1777005 BBa_K1777005]) based on this parts.When we planned to use this part to link our fusion component,we found it hard to design a proper primer to amplify it because of mutiple primer binding site as a result of tandem GGGS.Therefore, we first improved it by change the first GGGS to a natural linker obtained from linker database.In addition,the codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Such an improved GS linker could be easily assembled to your protein via overlap PCR and offer the property of nuclear localization. |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 12:00, 20 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1486003
Group: iGEM15_Fudan
Author: Xuhang Li
Summary:We developed a new part([http://https://parts.igem.org/Part:BBa_K1777005 BBa_K1777005]) based on this parts.When we planned to use this part to link our fusion component,we found it hard to design a proper primer to amplify it because of mutiple primer binding site as a result of tandem GGGS.Therefore, we first improved it by change the first GGGS to a natural linker obtained from linker database.In addition,the codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Such an improved GS linker could be easily assembled to your protein via overlap PCR and offer the property of nuclear localization.
User Reviews
UNIQ4bbd327c388a0bb0-partinfo-00000000-QINU UNIQ4bbd327c388a0bb0-partinfo-00000001-QINU