Revision as of 10:30, 20 September 2015

Pcons+B0034+FadL-GBP+B0030+GFP+J61048

Introduction

The transmembrane protein, FadL, fused with the GBP, can act as an anchoring motif for displaying GBP on the surface of E.coli. To achieve the convenient observation, the genetic sequence of green fluorescent protein was introduced in our design. With the dual display of the GBP and the GFP, the E.coli can not only bind on the gold surface but also produce green fluorescent signal.

Experiment

We intended to make the observation of the result more directly by producing green fluorescence at the same time. To make the comparison of the binding efficiency, we selected the genetic sequence, Pcons + rbs +GFP +ter, as the negative control group. Because there was no existence of fully-functioned phenomenon, there was no positive control in our experiment.

Fig.5(a)the results of the test group, which contains the genetic sequence of Pcon+ rbs + FadL-3rdGBP + rbs + GFP + ter

Fig.5(b)displays the consequence of the control group with the genetic sequence, Pcon+ rbs + GFP + ter.

According to the pictures shown above, we can see that there is more E.coli with gold-binding polypeptide being observed on the gold chip. It indicates that the E.coli with gold-binding polypeptide can attach on gold chip more effectively and efficiently than others without gold-binding polypeptide.

Sequence and Features