Difference between revisions of "Part:BBa K1694053"
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| − | + | <h1>'''Introduction'''</h1> | |
| + | To observe that our scFv can bind onto the cancer cells, we connected each scFv with different chromoprotein. Color proteins are commonly used as reporter gene for observation. However, chromoproteins can be conveniently observed by naked eye without the help of instruments. Therefore, when we conducted cell staining experiment, we can observe the binding distribution by color right after the experiments have finished. | ||
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Revision as of 10:01, 20 September 2015
Pcons+B0034+Lpp-OmpA-N+scFv(Anti-VEGF)+B0034+amilCP+B0015
Introduction
To observe that our scFv can bind onto the cancer cells, we connected each scFv with different chromoprotein. Color proteins are commonly used as reporter gene for observation. However, chromoproteins can be conveniently observed by naked eye without the help of instruments. Therefore, when we conducted cell staining experiment, we can observe the binding distribution by color right after the experiments have finished.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 451
Illegal NgoMIV site found at 1962 - 1000COMPATIBLE WITH RFC[1000]