Difference between revisions of "Part:BBa K1777019:Design"

 
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===Design Notes===
 
===Design Notes===
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when we chose a linker for our fusion protein，we would have wanted to use -gggsgggs- linker. But we found that such a tandem repeating linker is hard to work well with overlap PCR because the OE region should be unique. Therefore, we improved the original part by change the first GGGS to a carefully selected natural linker from linker database to avoid repeating sequence. Besides, we used the selected natural linker to adjust its length to about 50A.  
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When we chose a linker for our fusion protein，we would have wanted to use -gggsgggs- linker(BBa_K1486003). But we found that such a tandem repeating linker is hard to amplify via PCR because the primer binding site should be unique. Therefore, we improved the original part by change the first GGGS to a carefully selected natural linker from linker database[1] to avoid repeating sequence. Besides, we used the selected natural linker to adjust its length to about 50 Angstrom in total.
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===Source===
 
===Source===
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===References===
 
===References===
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1.linker database(http://www.ibi.vu.nl/programs/linkerdbwww/） George RA. and Heringa J. (2002) An analysis of protein domain linkers: their classification and role in protein folding. Protein Engineering 15, 871-879<br>
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2.[https://parts.igem.org/Part:BBa_K1486003 link  BBa_K1486003]

Latest revision as of 08:12, 20 September 2015


flexible linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When we chose a linker for our fusion protein，we would have wanted to use -gggsgggs- linker(BBa_K1486003). But we found that such a tandem repeating linker is hard to amplify via PCR because the primer binding site should be unique. Therefore, we improved the original part by change the first GGGS to a carefully selected natural linker from linker database[1] to avoid repeating sequence. Besides, we used the selected natural linker to adjust its length to about 50 Angstrom in total.

Source

BBa_K1486003

References

1.linker database(http://www.ibi.vu.nl/programs/linkerdbwww/） George RA. and Heringa J. (2002) An analysis of protein domain linkers: their classification and role in protein folding. Protein Engineering 15, 871-879
2.link BBa_K1486003