Difference between revisions of "Part:BBa K1648004:Design"

 
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===Design Notes===
 
===Design Notes===
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In order to form a homologous recombination template, we selected 250 bp of 5' and 3' ends from '''mamGFDC''', '''mamYXZ-ftsZ''' and '''mms6-mmsF-mms35-mms48''' ('''mamGC''', '''mamXY''' and '''mms6 operons''') except the mamC operon as flanking sequences. Fragments are joined via overlapping PCR.
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In order to introduce protein of interest to the magnetosome membrane, we add mamC in the front of multiple cloning sites of pSB1C3.
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[[File:K1648004_History.png|900px]]
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[[File:K1648004 history.png|800px]]
  
 
===Source===
 
===Source===

Latest revision as of 06:04, 20 September 2015

Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2759
    Illegal XbaI site found at 2765
    Illegal SpeI site found at 1
    Illegal PstI site found at 7
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2759
    Illegal SpeI site found at 1
    Illegal PstI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2759
    Illegal XhoI site found at 1162
    Illegal XhoI site found at 2188
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2759
    Illegal XbaI site found at 2765
    Illegal SpeI site found at 1
    Illegal PstI site found at 7
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2759
    Illegal XbaI site found at 2765
    Illegal SpeI site found at 1
    Illegal PstI site found at 7
    Illegal NgoMIV site found at 2546
    Illegal NgoMIV site found at 2636
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2611


Design Notes

In order to introduce protein of interest to the magnetosome membrane, we add mamC in the front of multiple cloning sites of pSB1C3.

K1648004 history.png

Source

Magnetospirillum gryphiswaldense MSR-1 v2, complete genome

References