Difference between revisions of "Part:BBa K1648006"

Revision as of 05:33, 20 September 2015

Insertion kit with GFP-nanobody

Consist of Insertion Kit(K1648004) and GFP-nanobody (K1648005) in order to fuse GFP-nanobody to a transmembrane protein on magnetosome, and express GFP-nanobody on magnetsome membrane for downstream applications.

GFP-nanobody will be expressed on the magnetosome after transformation of this part into A. vinelandii genome via random integration. Magnetosome with GFP-nanobodies can be applied in studying the level of expression of fused protein in Insertion Kit(K1648004) simply by measuring fluorescence intensity of magnetosome incubated with GFP beforehand.

The part is verified by double digestion(Fig 1.).

Fig 1. Checking of recombinant Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane (BBa_K1648004) using double digestion. L: DNA ladder. Lane 1-3: Insertion kit with GFP-nanobody (BBa_K1648006) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 [[File:NIKnano.jpg|400px]]
-Figure 1. Checking of recombinant Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane (BBa_K1648004) using double digestion. L: DNA ladder. Lane 1-3: Insertion kit with GFP-nanobody (BBa_K1648006) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site.
+Fig 1. Checking of recombinant Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane (BBa_K1648004) using double digestion. L: DNA ladder. Lane 1-3: Insertion kit with GFP-nanobody (BBa_K1648006) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site.
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