Difference between revisions of "Part:BBa K1648045:Experience"
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[[File:K1648045_pcr.png|250px|thumb|left|PCR product of K1648045:  The photo of 1% agarose gel electrophoresis. L: DNA ladder. Lane 1: PCR product of oprF encoding from the Azotobacter vinelandii strain DJ genome.
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[[File:K1648045_dd.png|350px|thumb|left|Double digestion of K1648045: L: DNA ladder. Lane 1-3: Recombination Template for pSB1C3-oprF(BBa_K1648045) with double digestion cut at EcoR1 and PstI sites, with single digestion at Pst1 site, without digestion.
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===Applications of BBa_K1648045===
 
===Applications of BBa_K1648045===
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An ongoing experiment will be carried out for the comparison between the transformed bacteria with [https://parts.igem.org/Part:BBa_K1648048 BBa_K1648048] for oprF from Azotobacter vinelandii [https://parts.igem.org/Part:BBa_K1648049 BBa_K1648049] for mutated oprF from Azotobacter vinelandii and [https://parts.igem.org/Part:BBa_K1648050 BBa_K1648050] for [https://parts.igem.org/Part:BBa_K1172501 BBa_K1172501] from Germany iGEM team. For comparison,an identical promoter, J13002, which is a constitutive promoter, is chosen to add before the gene in psb1C3 for constitutive expression of different OprF in bacteria.
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Provide an alternative choice of oprF.
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[[File:K1648048_dd.png|350px|thumb|right|Double digestion of K1648048:  L: DNA ladder. Lane 1-3: Recombination Template for J13002-oprF(BBa_K1648048) with double digestion cut at EcoR1 and PstI sites, with single digestion at Pst1 site, without digestion.]]
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===User Reviews===
 
===User Reviews===

Latest revision as of 05:17, 20 September 2015

PCR product of K1648045: The photo of 1% agarose gel electrophoresis. L: DNA ladder. Lane 1: PCR product of oprF encoding from the Azotobacter vinelandii strain DJ genome.
Double digestion of K1648045: L: DNA ladder. Lane 1-3: Recombination Template for pSB1C3-oprF(BBa_K1648045) with double digestion cut at EcoR1 and PstI sites, with single digestion at Pst1 site, without digestion.


















Applications of BBa_K1648045

Provide an alternative choice of oprF.

User Reviews

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