Difference between revisions of "Part:BBa K1648049:Experience"
 
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[[File:K1648049_dd.png|350px|thumb|left|Double digestion of K1648049:  L: DNA ladder. Lane 1-2: Recombination Template for R0040-oprF* (BBa_K1648049) with double digestion cut at EcoR1 and PstI sites, with single digestion at Pst1 site.]]
 
[[File:K1648049_dd.png|350px|thumb|left|Double digestion of K1648049:  L: DNA ladder. Lane 1-2: Recombination Template for R0040-oprF* (BBa_K1648049) with double digestion cut at EcoR1 and PstI sites, with single digestion at Pst1 site.]]
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===Applications of BBa_K1648049===
 
===Applications of BBa_K1648049===
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An ongoing experiment will be carried out for the comparison between the transformed bacteria with [https://parts.igem.org/Part:BBa_K1648048 BBa_K1648048] for oprF from Azotobacter vinelandii [https://parts.igem.org/Part:BBa_K1648049 BBa_K1648049] for mutated oprF from Azotobacter vinelandii and [https://parts.igem.org/Part:BBa_K1648050 BBa_K1648050] for [https://parts.igem.org/Part:BBa_K1172501 BBa_K1172501] from Germany iGEM team. For comparison,an identical promoter, J13002, which is a cons
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The ongoing experiment will be carried out for the comparison between the transformed bacteria with [https://parts.igem.org/Part:BBa_K1648048 BBa_K1648048] for oprF from Azotobacter vinelandii [https://parts.igem.org/Part:BBa_K1648049 BBa_K1648049] for mutated oprF from Azotobacter vinelandii and [https://parts.igem.org/Part:BBa_K1648050 BBa_K1648050] for [https://parts.igem.org/Part:BBa_K1172501 BBa_K1172501] from Germany iGEM team. For comparison,an identical promoter, J13002, which is a constitutive promoter, is chosen to add before the gene in psb1C3 for constitutive expression of different OprF in bacteria.
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===User Reviews===
 
===User Reviews===
 
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<!-- DON'T DELETE --><partinfo>BBa_K1648049 StartReviews</partinfo>

Latest revision as of 05:15, 20 September 2015

Double digestion of K1648049: L: DNA ladder. Lane 1-2: Recombination Template for R0040-oprF* (BBa_K1648049) with double digestion cut at EcoR1 and PstI sites, with single digestion at Pst1 site.


















Applications of BBa_K1648049

The ongoing experiment will be carried out for the comparison between the transformed bacteria with BBa_K1648048 for oprF from Azotobacter vinelandii BBa_K1648049 for mutated oprF from Azotobacter vinelandii and BBa_K1648050 for BBa_K1172501 from Germany iGEM team. For comparison,an identical promoter, J13002, which is a constitutive promoter, is chosen to add before the gene in psb1C3 for constitutive expression of different OprF in bacteria.

User Reviews

UNIQb767db94d6320c99-partinfo-00000000-QINU UNIQb767db94d6320c99-partinfo-00000001-QINU