Difference between revisions of "Part:BBa K1648049:Experience"

Latest revision as of 05:15, 20 September 2015

Double digestion of K1648049: L: DNA ladder. Lane 1-2: Recombination Template for R0040-oprF* (BBa_K1648049) with double digestion cut at EcoR1 and PstI sites, with single digestion at Pst1 site.

Applications of BBa_K1648049

The ongoing experiment will be carried out for the comparison between the transformed bacteria with BBa_K1648048 for oprF from Azotobacter vinelandii BBa_K1648049 for mutated oprF from Azotobacter vinelandii and BBa_K1648050 for BBa_K1172501 from Germany iGEM team. For comparison,an identical promoter, J13002, which is a constitutive promoter, is chosen to add before the gene in psb1C3 for constitutive expression of different OprF in bacteria.

User Reviews

UNIQab0ab772b6ec73eb-partinfo-00000000-QINU UNIQab0ab772b6ec73eb-partinfo-00000001-QINU

@@ Line 1: / Line 1: @@
 __NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+[[File:K1648049_dd.png|350px|thumb|left|Double digestion of K1648049:  L: DNA ladder. Lane 1-2: Recombination Template for R0040-oprF* (BBa_K1648049) with double digestion cut at EcoR1 and PstI sites, with single digestion at Pst1 site.]]
-how you used this part and how it worked out.
 ===Applications of BBa_K1648049===
+The ongoing experiment will be carried out for the comparison between the transformed bacteria with [https://parts.igem.org/Part:BBa_K1648048 BBa_K1648048] for oprF from Azotobacter vinelandii [https://parts.igem.org/Part:BBa_K1648049 BBa_K1648049] for mutated oprF from Azotobacter vinelandii and [https://parts.igem.org/Part:BBa_K1648050 BBa_K1648050] for [https://parts.igem.org/Part:BBa_K1172501 BBa_K1172501] from Germany iGEM team. For comparison,an identical promoter, J13002, which is a constitutive promoter, is chosen to add before the gene in psb1C3 for constitutive expression of different OprF in bacteria.
 ===User Reviews===