Difference between revisions of "Part:BBa K1648001"

Latest revision as of 05:08, 20 September 2015

Construct Map for K1648001

Recombination Template for mamXY, mamGC and mms Operons

Consist of six fragments from magnetosome island of Magnetospirillum gryphiswaldense genome.

Due to potential difficulty in introducing the large magnetosome formation operons into Azotobacter vinelandii, a "template" consists of flanking sequences from mamXY, mamGFDC and mms6 operons is designed for homologous recombination.

Part containing this template (K1648003) is introduced into A. vinelandii genome via random integration before full operon sequences to be transformed into A. vinelandii.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1333
Illegal BamHI site found at 432
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 603
Illegal NgoMIV site found at 785
Illegal NgoMIV site found at 875
Illegal NgoMIV site found at 1283
Illegal AgeI site found at 486
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 470
Illegal BsaI site found at 569
Illegal BsaI site found at 850

@@ Line 6: / Line 6: @@
 Due to potential difficulty in introducing the large magnetosome formation operons into ''Azotobacter vinelandii'', a "template" consists of flanking sequences from '''mamXY, mamGFDC and mms6 operons''' is designed for '''homologous recombination'''.
-Part containing this template ([https://parts.igem.org/Part:BBa_K1648002 K1648002]) is introduced into ''A. vinelandii'' genome via '''random integration''' before full operon sequences to be transformed into ''A. vinelandii''.
+Part containing this template ([https://parts.igem.org/Part:BBa_K1648003 K1648003]) is introduced into ''A. vinelandii'' genome via '''random integration''' before full operon sequences to be transformed into ''A. vinelandii''.
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