Difference between revisions of "Part:BBa K1676075"
 
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<partinfo>BBa_K1676075 short</partinfo>
 
<partinfo>BBa_K1676075 short</partinfo>
  
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This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the second base pair. The twelfth base pair A is substituted to T. The construct consist of the pLac promoter BBa_R0011, mutant RBS, eGFP BBa_E0040 and double terminator BBa_B0015. The purpose of this construct is to characterize the mutant RBS in Shewanella oneidensis MR1 strain.
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This reporter carries a mutant of RBS BBa_B0034. This mutant RBS carries a total of 7 base substitutions. This RBS mutants is mutated based on the results of our BBa_B0034 mutant library. Substitutions of basepairs with the highest GFP expression is carried out. The construct consist of the pLac promoter BBa_R0011, mutant RBS derived from BBa_B0034, eGFP BBa_E0040 and double terminator BBa_B0015. The purpose of this construct is to characterize the mutant RBS in Shewanella oneidensis MR1 strain.
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Previous results of mutant library:
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https://static.igem.org/mediawiki/2015/9/90/Sumary.png
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As we can see mutations on basepair 5, 6, 7, 8 and 9 brings to a lower GFP expression. Hence, no substitutions are made on these basepairs.
  
 
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Latest revision as of 02:57, 20 September 2015

GFP Reporter with Mutant RBS Opt

This reporter carries a mutant of RBS BBa_B0034. This mutant RBS carries a total of 7 base substitutions. This RBS mutants is mutated based on the results of our BBa_B0034 mutant library. Substitutions of basepairs with the highest GFP expression is carried out. The construct consist of the pLac promoter BBa_R0011, mutant RBS derived from BBa_B0034, eGFP BBa_E0040 and double terminator BBa_B0015. The purpose of this construct is to characterize the mutant RBS in Shewanella oneidensis MR1 strain.

Previous results of mutant library:

Sumary.png

As we can see mutations on basepair 5, 6, 7, 8 and 9 brings to a lower GFP expression. Hence, no substitutions are made on these basepairs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 725