Difference between revisions of "Part:BBa I746909:Experience"
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<h2> 2015 iGEM team Bielefeld-CeBiTec </h2>
 
<h2> 2015 iGEM team Bielefeld-CeBiTec </h2>
  
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<p> 2015 iGEM team Bielefeld-CeBiTec used this part and improved it by addition of a designed, translation enhancing 5'-untranslated region (5'-UTR; <a href="https://parts.igem.org/Part:BBa_K1758100">BBa_K1758100</a>). You can find further information at <a href="https://parts.igem.org/Part:BBa_K1758102">BBa_K1758102</a>). </p>   
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<p> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec">2015 iGEM team Bielefeld-CeBiTec</a> used this part and improved it by addition of a designed, translation enhancing 5'-untranslated region (5'-UTR; <a href="https://parts.igem.org/Part:BBa_K1758100">BBa_K1758100</a>). You can find further information at <a href="https://parts.igem.org/Part:BBa_K1758102">BBa_K1758102</a>). </p>   
  
 
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Revision as of 19:44, 19 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I746909

2015 iGEM team Bielefeld-CeBiTec

2015 iGEM team Bielefeld-CeBiTec used this part and improved it by addition of a designed, translation enhancing 5'-untranslated region (5'-UTR; BBa_K1758100). You can find further information at BBa_K1758102).

In vivo characterization of sfGFP with and without our designed, translation enhancing 5'-untranslated region (5'-UTR; BBa_K1758100). Relative fluorescence units were normalized on OD600. Error bars represent standard deviation of triplicates. PT7-sfGFP: BBa_I746909 (this part); PT7-UTR-sfGFP: BBa_K1758102


As can be seen in the picture, the difference was observable with the naked eye as well.


Comparision of cultures expressing sfGFP with the optimized UTR and without. Left: BBa_K1758102, right: BBa_I746909 (this part)



[http://2014.igem.org/Team:Imperial iGEM Team Imperial

IC-2014 glow1.jpg

IC-2014 glow2.jpg

We used this part as a template to make our own part (K1321337). We added Freiburg sites onto the existing sfGFP using overhang end primers during PCR. The part we made was sequence confirmed and showed very bright fluorescence, even when fused to our CBDs (cellulose binding domains).



[http://2014.igem.org/Team:Aachen iGEM Team Aachen

The iGEM Team Aachen used the biobrick I746909 to test their [http://2014.igem.org/Team:Aachen/Project/2D_Biosensor sensor chip technology].

Testing I746909 in sensor chips
I746909 in sensor chips induced with 2µl IPTG and measured with a plate reader. Top chip is not induced, bottom chip is induced with IPTG.

The biorbick I746909 was also used to test the measurement device [http://2014.igem.org/Team:Aachen/Project/Measurement_Device WatsOn].

Testing I746909 in WatsOn
I746909 in sensor chips induced with 2µl IPTG and measured with WatsOn. Left chip is not induced, right chip is induced with IPTG.





User Reviews

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