Difference between revisions of "Part:BBa K1676075"

Revision as of 17:08, 19 September 2015

GFP Reporter with Mutant RBS Opt

This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the second base pair. The twelfth base pair A is substituted to T. The construct consist of the pLac promoter BBa_R0011, mutant RBS, eGFP BBa_E0040 and double terminator BBa_B0015. The purpose of this construct is to characterize the mutant RBS in Shewanella oneidensis MR1 strain.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 725

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 __NOTOC__
 <partinfo>BBa_K1676075 short</partinfo>
-This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the second base pair. The TWELVETH base pair A is substituted to T. The construct consist of the pLac promoter BBa_R0011, mutant RBS, eGFP BBa_E0040 and double terminator BBa_B0015. The purpose of this construct is to characterise the mutant RBS in Shewanella Oneidensis MR1 strain.
+This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the second base pair. The twelfth base pair A is substituted to T. The construct consist of the pLac promoter BBa_R0011, mutant RBS, eGFP BBa_E0040 and double terminator BBa_B0015. The purpose of this construct is to characterize the mutant RBS in Shewanella oneidensis MR1 strain.
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