Difference between revisions of "Part:BBa K1383000:Experience"
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===Applications of BBa_K1383000=== | ===Applications of BBa_K1383000=== | ||
This part was amplified via PCR and inserted into a vector with a constitutive promoter. By expressing this mCherry gene with the native RBS in Methanococcus maripaludis we detected fluorescence with the use of a plate reader. | This part was amplified via PCR and inserted into a vector with a constitutive promoter. By expressing this mCherry gene with the native RBS in Methanococcus maripaludis we detected fluorescence with the use of a plate reader. | ||
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'''New Application of the Part''' | '''New Application of the Part''' | ||
| − | UGA-Georgia | + | <b>UGA-Georgia 2015 iGEM</b> |
| − | After development of this part along with BBa_K1383001 and BBa_K1383002, this part has been applied as a <b>standard</b> for our point mutation library. Rather than only being an expression tool for our developing library, it has become the sequence by which all our mutations are standardized against. | + | |
| + | After development of this part along with BBa_K1383001 and BBa_K1383002, this part has been applied as a <b>standard</b> for our point mutation library. Rather than only being an expression tool for our developing library, it has become the sequence by which all our mutations are standardized against. This can be seen in Table 1 where the fluorescence values are all percentages of relative native expression. | ||
[[Image:UGA-Georgia_Coverage_map_native.jpg|none|500px|]] | [[Image:UGA-Georgia_Coverage_map_native.jpg|none|500px|]] | ||
<b>UGA-Georgia 2015</b> Figure 6: This graph depicts point mutation versus percent of native sequence fluorescence. The native's full sequence is depicted along the x-axis at 100% fluorescence. Not all values shown here have been made into parts, the only part shown is the G to T point mutation at the 4th base site (BBa_K1635000). | <b>UGA-Georgia 2015</b> Figure 6: This graph depicts point mutation versus percent of native sequence fluorescence. The native's full sequence is depicted along the x-axis at 100% fluorescence. Not all values shown here have been made into parts, the only part shown is the G to T point mutation at the 4th base site (BBa_K1635000). | ||
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| + | [[Image:UGA-Georgia_Part_table.jpg|none|600px|]] | ||
| + | <b>UGA-Georgia 2015</b> Table 1: This table shows the percent of native expression for 8 different mutations of the native sequence as well as the native sequence. This table includes both pre-existing parts and what will become future parts. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 15:43, 19 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1383000
This part was amplified via PCR and inserted into a vector with a constitutive promoter. By expressing this mCherry gene with the native RBS in Methanococcus maripaludis we detected fluorescence with the use of a plate reader.
New Application of the Part
UGA-Georgia 2015 iGEM
After development of this part along with BBa_K1383001 and BBa_K1383002, this part has been applied as a standard for our point mutation library. Rather than only being an expression tool for our developing library, it has become the sequence by which all our mutations are standardized against. This can be seen in Table 1 where the fluorescence values are all percentages of relative native expression.
UGA-Georgia 2015 Figure 6: This graph depicts point mutation versus percent of native sequence fluorescence. The native's full sequence is depicted along the x-axis at 100% fluorescence. Not all values shown here have been made into parts, the only part shown is the G to T point mutation at the 4th base site (BBa_K1635000).
UGA-Georgia 2015 Table 1: This table shows the percent of native expression for 8 different mutations of the native sequence as well as the native sequence. This table includes both pre-existing parts and what will become future parts.
User Reviews
UNIQ20dfe12d658a7710-partinfo-00000000-QINU UNIQ20dfe12d658a7710-partinfo-00000001-QINU