Difference between revisions of "Part:BBa K1685011"
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| − | The biosynthesis of rhamnolipid is relatively simple. It only needs two enzymes which catalyse sequential reaction. The first enzyme, rhamnosyltransferase A (rhlA), which catalyse the dimerisation of two b-hydroxyacyl-ACP chains to make 3-(3-hydroxyalkanoyloxy)alkanoic acids (HAAs). The HAA will form the lipid moiety of the rhamnolipid. Rhamnosyltransferase B, rhlB, will then join TDP-l-rhamnose with HAA to produce mono-rhamnolipid. | + | <p>The biosynthesis of rhamnolipid is relatively simple. It only needs two enzymes which catalyse sequential reaction. The first enzyme, rhamnosyltransferase A (rhlA), which catalyse the dimerisation of two b-hydroxyacyl-ACP chains to make 3-(3-hydroxyalkanoyloxy)alkanoic acids (HAAs). The HAA will form the lipid moiety of the rhamnolipid. Rhamnosyltransferase B, rhlB, will then join TDP-l-rhamnose with HAA to produce mono-rhamnolipid.</p> |
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| + | <p> Because Escherichia coli can synthesize both rhamnose and fatty acid, the addition of these two enzymes will be sufficient to give the bacteria the ability to synthesize rhamnolipid.</p> | ||
Revision as of 10:30, 19 September 2015
Rhamnolipid production
This part encodes two enzymes, rhlA and rhlB (BBa_K653000), for biosynthesis of rhamnolipid. The enzymes are under T7 promoter with medium strengh and a strong ribosomal binding site (BBa_K613010), and stopped with a double terminator (BBa_B0015).
The promoter had the second highest efficiency as shown by EPFL team 2011 (https://parts.igem.org/Part:BBa_K613010:Experience).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 225
Illegal BamHI site found at 785
Illegal XhoI site found at 961
Illegal XhoI site found at 2247 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1140
Illegal NgoMIV site found at 1861
Illegal NgoMIV site found at 1974 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 450
Illegal BsaI site found at 1490
Illegal BsaI.rc site found at 634
Usage and Biology
The biosynthesis of rhamnolipid is relatively simple. It only needs two enzymes which catalyse sequential reaction. The first enzyme, rhamnosyltransferase A (rhlA), which catalyse the dimerisation of two b-hydroxyacyl-ACP chains to make 3-(3-hydroxyalkanoyloxy)alkanoic acids (HAAs). The HAA will form the lipid moiety of the rhamnolipid. Rhamnosyltransferase B, rhlB, will then join TDP-l-rhamnose with HAA to produce mono-rhamnolipid.
Because Escherichia coli can synthesize both rhamnose and fatty acid, the addition of these two enzymes will be sufficient to give the bacteria the ability to synthesize rhamnolipid.