Difference between revisions of "Part:BBa K1676037"
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<partinfo>BBa_K1676037 short</partinfo> | <partinfo>BBa_K1676037 short</partinfo> | ||
This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the second base pair. The second base pair A is substituted to C. | This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the second base pair. The second base pair A is substituted to C. | ||
− | The construct consist of the pLac promoter BBa_R0011, mutant RBS, eGFP BBa_E0040 and double terminator BBa_B0015. | + | The construct consist of the pLac promoter BBa_R0011, mutant RBS derive from BBa_B0034 , eGFP BBa_E0040 and double terminator BBa_B0015. |
The purpose of this construct is to characterise the mutant RBS in Shewanella Oneidensis MR1 strain. | The purpose of this construct is to characterise the mutant RBS in Shewanella Oneidensis MR1 strain. | ||
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+ | This reporter can also be derived from the existing construct, BBa_J69511. | ||
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+ | The characterisation is shown below, | ||
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+ | Growth curve: | ||
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+ | https://static.igem.org/mediawiki/2015/1/1f/123_gc.png | ||
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+ | GFP Reading: | ||
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+ | https://static.igem.org/mediawiki/2015/8/81/123.png | ||
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+ | It is shown to have a fluorescence reading higher than the wild type RBS reporter, BBa_J69511. | ||
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Revision as of 09:43, 19 September 2015
GFP Reporter with Mutant RBS 1AC
This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the second base pair. The second base pair A is substituted to C. The construct consist of the pLac promoter BBa_R0011, mutant RBS derive from BBa_B0034 , eGFP BBa_E0040 and double terminator BBa_B0015.
The purpose of this construct is to characterise the mutant RBS in Shewanella Oneidensis MR1 strain.
This reporter can also be derived from the existing construct, BBa_J69511.
The characterisation is shown below,
Growth curve:
GFP Reading:
It is shown to have a fluorescence reading higher than the wild type RBS reporter, BBa_J69511.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 725