Difference between revisions of "Part:BBa K1758323"

Revision as of 05:52, 19 September 2015

UTR-sfGFP under control of Copper responsive promoter

Copper induceble promoter with an untranslated region and sfGFP for detection via fluorescence

Usage and Biology

CopAP is the central component in obtaining copper homeostasis, which exports free copper from cytoplasm to the periplasm. This is enabled by copper induced activation of the operon transcription via CueR. The CueR-Cu+ is the DNA-binding transcriptional dual regulator which activates transcription (Yamamoto, Ishihama 2005).

In our project this part is essential for the in vitro characterization of our copper sensor. We started characterizing it with this device, but data suggested that we could reach higher fluorescence level using a T7 promoter, which was realized in BBa_K1758325.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 29
Illegal SapI.rc site found at 179

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 ===Usage and Biology===
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+CopAP is the central component in obtaining copper homeostasis, which exports free copper from cytoplasm to the periplasm. This is enabled by copper induced activation of the operon transcription via CueR. The CueR-Cu+ is the DNA-binding transcriptional dual regulator which activates transcription (Yamamoto, Ishihama 2005).
 <p>In our project this part is essential for the <i> in vitro </i> characterization of our copper sensor. We started characterizing it with this device, but data suggested that we could reach higher fluorescence level using a T7 promoter, which was realized in <a href="https://parts.igem.org/Part:BBa_K1758325" target="_blank">BBa_K1758325</a>.</p>
 </html>